Cryostat Microtomy of Lung Tissue in an Expanded State
- 1 January 1975
- journal article
- Published by Taylor & Francis in Stain Technology
- Vol. 50 (1) , 43-45
- https://doi.org/10.3109/10520297509117030
Abstract
A technique is reported for cryostat sectioning of lung tissue in an expanded state for use in viral immunofluorescence studies. A 1: 2 mixture of O.C.T. embedding compound and phosphate-buffered saline is injected intratracheally into fresh lung tissne. The lung tissue is frozen in liquid nitrogen and sectioned with a cryostat. Compared to other published reports of lung sectioning for immunofluorescence miscroscopy, this method has the advantages of bekg easy and quick, maintaining the lung sectiom in an expanded rather than collapsed state and avoiding contact with chemicals potentially capable of altering sensitive viral antigens.Keywords
This publication has 1 reference indexed in Scilit:
- A PARAFFIN EMBEDDING TECHNIQUE FOR STUDIES EMPLOYING IMMUNOFLUORESCENCEJournal of Histochemistry & Cytochemistry, 1962