Characterization of protein S, a .gamma.-carboxyglutamic acid containing protein from bovine and human plasma
- 6 March 1979
- journal article
- research article
- Published by American Chemical Society (ACS) in Biochemistry
- Vol. 18 (5) , 899-904
- https://doi.org/10.1021/bi00572a026
Abstract
Protein S is a vitamin K dependent protein of unknown function, which is present in mammalian plasma. It was isolated from bovine plasma by barium citrate adsorption and elution, ammonium sulfate fractionation and column chromatography on DEAE-Sephadex, heparin-agarose and polyhomoarginine-Sepharose. Bovine Protein S (MW 64,200) is a single-chain glycoprotein with an amino-terminal sequence of Ala-Asn-Thr-Leu-Leu-. It contains 7.0% carbohydrate and 10 residues of .gamma.-carboxyglutamic acid/mol of protein. Human Protein S (MW 69,000) is also a single-chain glycoprotein with an amino-terminal sequence of Ala-Asn-Ser-Leu-Leu-. It contains 7.8% carbohydrate and 10 residues of .gamma.-carboxyglutamic acid/mol of protein. Protein S from bovine or human plasma apparently shows many similarities to the other vitamin K dependent proteins present in plasma.This publication has 19 references indexed in Scilit:
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