Evidence for a second “Prereplicative G2” repair mechanism, specific for γ-induced damage, in wild-type Schizosaccharomyces pombe
- 1 January 1977
- journal article
- conference paper
- Published by Springer Nature in Molecular Genetics and Genomics
- Vol. 154 (2) , 129-133
- https://doi.org/10.1007/bf00330828
Abstract
The major part of the substantial γ-resistance of wild-type Schizosaccharomyces pombe appears to be due to prereplicative recombinational repair mechanisms. The existence of a second “prereplicative G2” repair pathway, specific for γ-induced damage, has now been deduced from studies of the effect of the repair inhibitor caffeine on γ-irradiated G1 phase and G2 phase cells. Only G2 cells are additionally inactivated on exposure to caffeine after γ-irradiation. This shows that both known caffeine-sensitive γ-repair processes (Gentner and Werner, Molec. gen. Genet. 145, 1–5 [1976]) are dependent on the presence of a duplicated genome (2c) at the time of radiation exposure. Pathway I is the known “prereplicative G2” repair process (Fabre, Radiation Res. 56, 528–539 [1973]) which is involved in both UV- and γ-repair, and which requires post-irradiation protein synthesis for activity. Pathway II represents a second distinct “prereplicative G2” repair mechanism; it differs from the first in that it is specific for repair of γ-induced damage and appears to be constitutive.This publication has 23 references indexed in Scilit:
- Slow UV-recovery and fast γ-recovery in wild-type Schizosaccharomyces pombeMolecular Genetics and Genomics, 1977
- Repair in Schizosaccharomyces pombe as measured by recovery from caffeine enhancement of radiation-induced lethalityMolecular Genetics and Genomics, 1975
- Excision of pyrimidine dimers by several UV-sensitive mutants of S. pombeMolecular Genetics and Genomics, 1975
- Dark repair inhibitors and pathways for repair of radiation damage in Schizosaccharomyces pombeMolecular Genetics and Genomics, 1974
- The Role of Repair Mechanisms in the Variations of Ultraviolet and γ-Radiation Sensitivity during the Cell Cycle of Schizosaccharomyces pombeRadiation Research, 1973
- Relation between repair mechanisms and induced mitotic recombination after UV irradiation, in the yeast Schizosaccharomyces pombeMolecular Genetics and Genomics, 1972
- Mosaicism and lethal sectoring in G1 cells of Schizosaccharomyces pombeMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1971
- UV-sensitivity of the wild-type and different UVS mutants of schizosaccharomyces pombe Influence of growth stages and DNA content of the cellsMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1970
- DNA synthesis in the fission yeast Schizosaccharomyces pombeExperimental Cell Research, 1970
- The production, by nitrous acid, of complete and mosaic mutations during defined nuclear stages in cells of Schizosaccharomyces pombeMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1970