Glucose metabolism in Candida species

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Abstract
Nineteen cultures representing not less than 6 species of Candida grew well in simple media of inorganic salts and glucose, with either ammonium phosphate or ammonium sulphate as sources of N, and all the strains formed: (a) pyruvic acid, (b) another organic acid (or acids) and (c) ethanol, which in some cases was detected in traces only. Candida spp. were unable to utilize nitrates as source of N, and were restricted in their growth and acid-forming capacity by addition of nitrite to the medium. When thiamine hydrochloride (100 [mu]g/100 ml) was added to cultures of C. krusei, which normally produces an appreciably large yield of ethanol from glucose, the rate of utilization of glucose was increased, accumulation of pyruvic acid was prevented and the yield of ethanol was greater than that afforded by the control without added thiamine. Addition of thiamine hydro-chloride (100 [mu]g/100 ml) to cultures of the very weakly fermentative C. mycoderma caused an increase in the rate of utilization of glucose and prevented accumulation of pyruvic acid, but did not promote formation of ethanol. Addition of sodium arsenite (0.002[image]) to cultures of C. krusei caused the rate of utilization of glucose to fall and the yield of ethanol to be substantially increased.

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