Changes in Intracellular pH Are Not Correlated with the Circadian Rhythm of Neurospora

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Abstract
Homogenates of the endosperm of castor bean (Ricinus communis var. Hale) were prepared at intervals during germination and fractionated on sucrose gradients. Early in germination when glyoxysomes were being produced, a substantial proportion (50%) of the activities of malate synthetase and citrate synthetase was recovered in the membranes of the endoplasmic reticulum (mean density 1.12 grams per cubic centimeter). This proportion declined to less than 10% at 4 days when the glyoxysomes were fully developed.