Transcriptional regulation of the yeast cytochrome c gene.

Abstract
DNA from the cloned yeast iso-1-cytochrome c, cyc1 gene was used in a hybridization assay to measure levels and rates of synthesis of cyc1 RNA. Derepressed cells synthesized cyc1 RNA at 6 times the rate of that of glucose-repressed cells. Upon glucose addition to a derepressed culture, the transcription of the cyc1 gene was repressed within 2.5 min. The half-life of hybridizable cyc1 RNA was 12-13.5 min under repressed and derepressed conditions and during repression. The expression of the cyc1 gene is apparently subject to transcriptional regulation.