Immunological Evidence for the Presence of Ribulose Bisphosphate Carboxylase in Guard Cell Chloroplasts

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Abstract
Ribulose bisphosphate carboxylase (Rubisco) was found in Vicia faba L. guard cell chloroplasts by 2 immunological methods, using antibodies raised against highly purified subunits of ribulose bisphosphate carboxylase. Indirect cytoimmunofluorescence revealed binding of antibodies against both the small and large subunits of ribulose bisphosphate carboxylase. Binding was observed only after partial digestion of guard cell walls by 4% Cellylysin to facilitate antibody penetration. After electrophoresis of a homogenate of guard cell protoplasts, the presence of both subunits was also revealed by immunolabeling technique. Positive response required the inhibition of proteolysis which appeared to be active upon homogenization.