Altered microtubule organization in small-calibre axons of mice lacking tau protein

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Abstract
THE tau gene encodes a protein (Tau) that is a major neuronal microtubule-associated protein localized mostly in axons1–4. It has microtubule-binding and tubulin-polymerizing activity in vitro 3,4 and is thought to make short crossbridges between axonal microtubules5,6. Further, tau-transfected non-neuronal cells extend long axon-like processes in which microtubule bundles resembling those in axons are formed6–8. In contrast, tau antisense oligo-nucleotides selectively suppress axonal elongation in cultured neurons9,10. Thus tau is thought to be essential for neuronal cell morphogenesis, especially axonal elongation and maintenance. To test this hypothesis, we used gene targeting to produce mice lacking the tau gene. We show that the nervous system of fan-deficient mice appears to be normal immunohistologically. Furthermore, axonal elongation is not affected in cultured neurons. But in some small-calibre axons, microtubule stability is decreased and microtubule organization is significantly changed. We observed an increase in microtubule-associated protein 1A which may compens-ate for the functions of tau in large-calibre axons. Our results argue against the suggested role of tau in axonal elongation but confirm that it is crucial in the stabilization and organization of axonal microtubules in a certain type of axon.