The Molecular Classes of Two Homocytotropic Antibodies in the Mouse

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Abstract
Heat-labile homocytotropic antibody (HCA), which persists in homologous skin sites for long periods of time, and heat-stable HCA, which persists in skin sites for short periods of time, were studied to determine their immunoglobulin classes. Absorption was done in liquid phase with monospecific antisera against mouse IgG2, IgG3, IgM and IgA, and with a Sepharose-coupled insoluble anti-IgG1. The short-latency, heat-stable HCA was completely removed with anti-IgG1 and by none of the other absorptions, i.e., it is an IgG1 antibody. The long-latency, heat-labile HCA was not removed by any of the immunoabsorptions. In addition, neither of the mouse HCA was removed from immune sera by absorption with a potent anti-human IgE antiserum, and fixation of mouse HCA to mouse skin was not blocked by human IgE Fc at 10 µ/ml concentration. The long-latency HCA is similar in many properties to human IgE and is accordingly tentatively called mouse “IgE” antibody. However, immunologic cross-reactivity between mouse and human IgE could not be demonstrated, nor could fixation of mouse IgE to mouse skin be blocked by human IgE Fc.

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