Inactivation of delayed outward current in molluscan neurone somata.

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Abstract
Inactivation of delayed outward current was studied by voltage clamp of isolated neuron somata of the mollusk Archidoris mantereyensis and Anisodoris nobilis. During prolonged voltage clamp steps in normal artificial seawater delayed outward current rises to a peak and declines to a non-zero steady state. During repetitive clamp pulses at repetition rates slower than 2/s, the amplitude of peak outward current in the 2nd pulse is commonly less than the amplitude at the end of the preceding pulse, giving the impression of continued inactivation during the repolarized interval. This property is termed cumulative inactivation. Two components of delayed outward current were separated using tetraethyl ammonium ions (TEA) and Co. External TEA blocks 90% of a voltage and time dependent outward current termed K current (IK). External Co blocks 85% of a Ca activated delayed outward current termed Ca current (ICa does not inactivate during prolonged or repetitive voltage clamp pulses. IK inactivates during prolonged voltage clamp steps and shows cumulative inactivation during repetitive voltage clamp pulses. Inactivation of IK is voltage and time dependent and does not require influx of Ca ions. As measured by a prepulse method, the onset of inactivation is characterized by a 2 time constant process. Fast inactivation occurs with a time course comparable to the rate of rise of outward current and can account for 90% of total inactivation. Recovery from inactivation is slow with a time constant approximately an order of magnitude slower than the onset of inactivation. The current-voltage (I-V) curve for peak IK can be N-shaped, with a region of negative slope resistance in the range of +30-+80 mV. The I-V curve for steady state IK, shows little or no tendency to form a local maximum. The pattern of delayed outward current varies considerably between cells. A major contributing factor to this variability appears the relative contributions of ICa and IK to delayed outward current.