CHARACTERIZATION OF HUMAN AND MOUSE C-FES CDNA CLONES AND IDENTIFICATION OF THE 5' END OF THE GENE
- 1 March 1990
- journal article
- research article
- Vol. 5 (3) , 267-275
Abstract
CDNA clones of the human c-fes mRNA were isolated. Nucleotide analysis showed that c-fes mRNA contains a 2514 nucleotide open reading frame, which could encode for a 93 kDa preotin, and both 5'' and 3'' v-fes non-homologous sequences. Primer extension experiments confirmed that the longest isolated cDNAs are about the same length as the entire human c-fes mRNA. Sequence comparison between human c-fes cDNA and the corresponding genomic regions identified a 5'' viral-non homologous exon (exon 1) located 491 bp upstream from the first v-fes homologous exon. The genomic region surrounding c-fes exon 1 contains CpG island and acts as a promoter in vitro. Analysis of the 5'' end of mouse c-fes cDNA suggested that the 5'' human and mouse gene structure are similar.This publication has 6 references indexed in Scilit:
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