Confocal scanning laser microscopy of mitochondria: a possible tool in the diagnosis of mitochondrial disorders
- 21 March 1990
- journal article
- Published by Wiley in Journal of Inherited Metabolic Disease
- Vol. 14 (1) , 45-48
- https://doi.org/10.1007/bf01804387
Abstract
This paper describes a non-invasive method for the study of mitochondrial morphology in cultured human skin fibroblasts by confocal scanning laser microscopy after staining the mitochondria with 2-[4-(dimethylaminostyryl]-1-methylpyridinium iodide. This method is applied to compare mitochondria in fibroblasts from healthy individuals and from patients with mitochondrial myopathy. In most cases there is a striking swelling of the patient's mitochondria and a loss of fine structure. The results with respect to the potential of this method as a diagnostic tool are discussed.Keywords
This publication has 5 references indexed in Scilit:
- STUDIES ON THE FORMATION OF LACTATE AND PYRUVATE FROM GLUCOSE IN CULTURED SKIN FIBROBLASTS - IMPLICATIONS FOR DETECTION OF RESPIRATORY-CHAIN DEFECTS1989
- Morphological observations in skeletal muscle from patients with a mitochondrial myopathyJournal of Inherited Metabolic Disease, 1987
- Fluorimetry of mitochondria in cells vitally stained with DASPMI or rhodamine 6 GOCell Biochemistry and Function, 1983
- Dimethylaminostyrylmethylpyridiniumiodine (DASPMI) as a fluorescent probe for mitochondria in situBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1976