Cell cycle stage-specific accumulation of mRNAs encoding tubulin and other polypeptides in Chlamydomonas.

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Abstract
The accumulation pattern of a number of forms of mRNA during the cell cycle of Chlamydomonas was examined by 2 dimensional gel analysis of in vitro translation products and by RNA blot hybridization analysis. Two-dimensional gel analysis revealed that 10-15% of the 300 most abundant translation products are differentially synthesized from RNA obtained at various cell cycle stages. RNA forms that direct the synthesis of .alpha.- and .beta.-tubulins and that hybridize to cloned .alpha.- and .beta.-tubulin probes accumulate coordinately during the predivision period of the cell cycle, reaching peak levels before or during division. Other forms of RNA represented by selected cloned cDNA probes undergo a number of different cell cycle patterns of accumulation. The accumulation patterns of these forms of RNA are not directly influenced by ongoing illumination conditions, even though alternating light-dark illumination cycles are used to synchronize Chlamydomonas cells. Apparently there may be a complex program of gene expression correlated with cell cycle progression in Chlamydomonas.