Inhibition by viomycin of cell-free protein synthesis in sensitive and resistant strains of Rhizobium meliloti
- 1 November 1969
- journal article
- Published by Canadian Science Publishing in Canadian Journal of Biochemistry
- Vol. 47 (11) , 1092-1094
- https://doi.org/10.1139/o69-174
Abstract
Viomycin at a concentration of 2–10 μg/ml inhibited cell-free polypeptide synthesis in both a viomycin-sensitive and a viomycin-resistant strain of Rhizobium meliloti when either natural messenger or polyuridylic acid (poly U) was employed. Consequently, viomycin resistance in the resistant bacterium is not the result of an inherently resistant protein-synthesizing system or of an intracellular modification of the viomycin molecule. The antibiotic did not prevent the formation of active poly U – ribosome complexes, but did inhibit the passage of 14C from 14C-phenylalanine-charged transfer RNA to these complexes.Keywords
This publication has 0 references indexed in Scilit: