Differential expression and functional analysis of three calmodulin isoforms in germinating pea (Pisum sativum L.) seeds

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Abstract
Implication of the ubiquitous, highly conserved, Ca2+ sensor calmodulin (CaM) in pea seed germination has been investigated. Mass spectrometry analysis of purified CaM revealed the coexistence in seeds of three protein isoforms, diverging from each other by single amino acid substitution in the N‐terminal α‐helix. CaM was shown to be encoded by a small multigenic family, and full‐length cDNAs of the three isoforms (PsCaM1, 2 and 3) were isolated to allow the design of specific primers in more divergent 5′ and 3′ untranslated regions. Expression studies, performed by semiquantitative RT–PCR, demonstrated differential expression patterns of the three transcripts during germination. PsCaM1 and 2 were detected at different levels in dry axes and cotyledons, and they accumulated during imbibition and prior to radicle protrusion. In contrast, PsCaM3 appeared only upon radicle protrusion, then gradually increased in both tissues. To characterise the biochemical properties of the CaM isoforms, functional analyses were conducted in vitro using recombinant Strep‐tagged proteins (CaM1‐ST, CaM2‐ST and CaM3‐ST) expressed in Escherichia coli. Gel mobility shift assays revealed that CaM1‐ST exhibited a stoichiometric binding of a synthetic amphiphilic CaM kinase II peptide while CaM2‐ST and CaM3‐ST affinities for the same peptide were reduced. Affinity differences were also observed for CaM isoform binding to Trp‐3, an idealised helical CaM‐binding peptide. However, the three proteins activated in the same way the CaM‐dependent pea NAD kinase. Finally, the significance of the single substitutions upon CaM interaction with its targets is discussed in a structural context.

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