Novoblocin inhibits interactions required for yeast TFIIIB sequestration during stable transcription complex formationin vitro
- 1 January 1987
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 15 (4) , 1493-1506
- https://doi.org/10.1093/nar/15.4.1493
Abstract
Novobiocin concentrations normally used to inhibit a putative eukaryotic DNA gyrase have been found to inhibit transcription of a yeast 5S rRNA gene using an in vitro yeast transcription system. Purified RNA polymerase III and three yeast transcription factors (chromatographically separated, partially purified and free of any detectable gyrase activity) were used. Novobiocin prevents specific transcription if added to the in vitro system immediately prior to the addition of transcription factors and RNA polymerase. If a stable transcription factor complex is allowed to form prior to the addition of novobiocin, concentrations of novobiocin as high as 1000 micrograms/ml have no effect on in vitro transcription. Transcription factors TFIIIA and TFIIIC are able to be stably sequestered onto 5SrDNA-cellulose, but factor TFIIIB is not able to associate with the 5SrDNA-TFIIIA-TFIIIC complex in the presence of novobiocin. Although novobiocin is able to precipitate other basic proteins, it does not appear to precipitate any of these class III gene transcription factors, but instead appears to act by disrupting specific factor-factor interactions.Keywords
This publication has 20 references indexed in Scilit:
- Gyration is required for 5S RNA transcription from a chromatin template.Proceedings of the National Academy of Sciences, 1986
- A bacteriophage RNA polymerase transcribes through a Xenopus 5S RNA gene transcription complex without disrupting itCell, 1986
- Novobiocin precipitates histones at concentrations normally used to inhibit eukaryotic type II topoisomeraseNucleic Acids Research, 1986
- The positive transcription factor of the 5S RNA gene induces a 5S DNA-specific gyration in xenopus oocyte extractsCell, 1985
- An ATP-dependent supercoiling topoisomerase ofChlamydomonas reinhardtiiaffects accumulation of specific chforoplast transcriptsNucleic Acids Research, 1985
- Formation of a rate-limiting intermediate in 5S RNA gene transcriptionCell, 1985
- Chromatin assembly in Xenopus oocytes: In vivo studiesCell, 1984
- Stable transcription complexes of Xenopus 5S RNA genes: A means to maintain the differentiated stateCell, 1982
- DNA gyrase: subunit structure and ATPase activity of the purified enzyme.Proceedings of the National Academy of Sciences, 1978
- Energy coupling in DNA gyrase and the mechanism of action of novobiocinProceedings of the National Academy of Sciences, 1978