KINETICS OF DOUBLE-ANTIBODY RADIOIMMUNOASSAY SYSTEMS FOR HUMAN GONADOTROPHINS
- 1 November 1970
- journal article
- Published by Oxford University Press (OUP) in Acta Endocrinologica
- Vol. 65 (3) , 453-458
- https://doi.org/10.1530/acta.0.0650453
Abstract
Incubation time curves were investigated in double-antibody radioimmunoassay systems. It was confirmed that the first reaction is not always halted on addition of the second antiserum; depending on the condition of the system at the time of adding the second antiserum, the first reaction may continue slowly, come to a standstill, or be reversed. A kinetic interpretation is discussed. Under the experimental conditions employed, the distortion of incubation time curves produced by disregarding the observed phenomena was found to be slight.Keywords
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