Reagents for the site-specific cleavage of megabase DNA
- 1 September 1992
- journal article
- Published by Springer Nature in Nature
- Vol. 359 (6390) , 87-88
- https://doi.org/10.1038/359087a0
Abstract
The physical mapping of chromosomes will be facilitated by methods of breaking large DNA into manageable fragments, or cutting uniquely at genetic markers of interest. Key issues in the design of sequence-specific DNA cleaving reagents are the specificity of binding, the number of different sequences that can be targeted and the cleavage yield.Keywords
This publication has 18 references indexed in Scilit:
- Analysis of promoter-specific repression by triple-helical DNA complexes in a eukaryotic cell-free transcription systemBiochemistry, 1992
- Site-Specific Cleavage of Human Chromosome 4 Mediated by Triple-Helix FormationScience, 1991
- NMR structural studies of intramolecular (Y+)n.cntdot.(R+)n(Y-)n DNA triplexes in solution: imino and amino proton and nitrogen markers of G.cntdot.AT base triple formationBiochemistry, 1991
- Second Structural Motif for Recognition of DNA by Oligonucleotide-Directed Triple-Helix FormationScience, 1991
- NMR studies of triple-strand formation from the homopurine-homopyrimidine deoxyribonucleotides d(GA)4 and d(TC)4Biochemistry, 1989
- Recognition of Thymine Adenine Base Pairs by Guanine in a Pyrimidine Triple Helix MotifScience, 1989
- Site-Specific Oligonucleotide Binding Represses Transcription of the Human c- myc Gene in VitroScience, 1988
- Sequence-Specific Cleavage of Double Helical DNA by Triple Helix FormationScience, 1987
- Sequence-specific recognition, photocrosslinking and cleavage of the DNA double helix by an oligo-(α]-thymidylate covalently linked to an azidoproflavine derivativeNucleic Acids Research, 1987
- FORMATION OF A THREE-STRANDED POLYNUCLEOTIDE MOLECULEJournal of the American Chemical Society, 1957