Biochemical Methods for Automated Bacterial Identification and Testing Metabolic Activities in Water and Wastewater

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Abstract
The improvements of computer-assisted identification were used to develop a new microplate system for characterization and identification of various gram-negative and gram-positive heterotrophic bacteria from the environment. In standard microtitration plates about 90 biochemical tests, some of them conventional tests, carbon substrate assimilation tests and enzyme tests using chromogenic substrates can be performed. Reading of the test results is done automatically by a photometer coupled to a computer. The applications of this identification system are shown by two waste water samples.

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