BOVINE LYMPHOCYTES - ENHANCED E-ROSETTE FORMATION AFTER STORAGE OR GRADIENT CENTRIFUGATION

  • 1 January 1977
    • journal article
    • research article
    • Vol. 29  (2) , 304-310
Abstract
Incubation of bovine thymus lymphocytes with SRBC [sheep red blood cells (E)] for .gtoreq. 1 h at 0.degree. C during the E-rosette test gave higher rosette counts than tests receiving no such incubation. Further enhancement was seen if lymphocytes were stored in fetal calf serum (FCS) or BSA [bovine serum albumin] solutions at 4.degree. C for 18 h prior to rosette tests. Best enhancement due to storage in BSA was seen when cells were subsequently tested in FCS. After storage in BSA many rosettes occurred when tests were done in BSA, but few rosettes occurred in BSA without prior storage. Less enhancement was seen after storage in sodium metrizoate (NaM). Enhancement in FCS and 10% BSA occurred after 1 day, but not .gtoreq. 2 days of storage at 4.degree. C, but was not seen when cells were stored at 37.degree. C. Rosette formation was about doubled by centrifugation of lymphocytes through BSA gradients prior to rosette tests; centrifugation through NaM gradients had no such effect. Rosette formation is apparently a property of an immature bovine T [thymus-derived] cell; on storage some cells probably develop and subsequently lose ability to form rosettes.