A Met-Enkephalin Analog Inhibits Adrenocorticotropin Secretion by Cultured Pituitary Cells from a Patient with Nelson's Syndrome*
- 1 November 1981
- journal article
- research article
- Published by The Endocrine Society in Journal of Clinical Endocrinology & Metabolism
- Vol. 53 (5) , 1084-1086
- https://doi.org/10.1210/jcem-53-5-1084
Abstract
ACTH secretion by cultured nonenzymatically dispersed pituitary tumor cells from a patient with Nelson's syndrome was studied. Hormone release was suppressed by 74 ± 6% by the addition of 1 /XM of the met-enkephalin analog FK 33824, while naloxone (1 /XM) stimulated ACTH release by 70 ± 5%. Somatostatin, dexamethasone, bromocriptine, and cyproheptadine in a concentration of 1 /AM each inhibited ACTH release by 25 ± 2%, 35 ± 2%, 52 ± 2%, and 61 ± 4%, respectively, while lysine vasopressin (0.1 /IM) and dibutyryl cAMP (5 mM) stimulated ACTH release by 112 ± 8% and 220 ± 4%, respectively. In conclusion, it was shown that the stimuli mentioned above directly affect ACTH secretion by the pituitary tumor cells. The inhibitory action of the met-enkephalin analog and the stimulatory action of naloxone on ACTH secretion make the presenceof opiate receptors on this type of tumor likely.Keywords
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