Notes: Separation of 13-and 9-Hydroperoxide Lyase Activities in Cotyledons of Cucumber Seedlings
Open Access
- 1 October 1989
- journal article
- Published by Walter de Gruyter GmbH in Zeitschrift für Naturforschung C
- Vol. 44 (9-10) , 883-885
- https://doi.org/10.1515/znc-1989-9-1031
Abstract
In cucumber cotyledons, both C6 - and C9- aldehyde were formed via hydroperoxide (HPO) lyase activity. Because it has not been elucidated whether these activities are attributed to one enzyme which can cleave both 13-and 9-HPO or to two or more enzymes each of which specifically cleaves 13-or 9-HPO , an attempt to separate HPO lyase activity was done. Ion exchange chromatography separated this activity into two fractions, one of which specifically cleaved 13-hydroperoxylinoleic acid and the other specifically cleaved the 9-isomer. 13-HPO-specific activity was most active at pH 8.0 and 9-HPO-specific one was at pH 6.5. SH -reagents inhibited both the lyases but to different extents.Keywords
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