Use of the Nonimmersed in Vitro Chicken Tracheal Preparation for the Study of Ciliary Transport Activity Cigarette Smoke and Related Components

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Abstract
A bioassay has been developed and used to quantitatively characterize the effects of cigarette smoke on ciliary function. Diluting smoke from 50% (40 ml) to 3% (2.5 ml) increases the number of four-second exposures required for 90% to 100% inhibition from 8 to 80 (or more) puffs. A high degree of correlation and notable similarity in slope of dose-response curves was found for regular cigarettes, cellulose acetate filter cigarettes, and cigarettes with filters of cellulose acetate with activated carbon. A 50% reduction (dose required to inhibit particle transport rate to 50% of control rate [ED50]) in transport activity was observed with 8 ml (one to ten dilution) of nonfiltered cigarette smoke after eight exposures of four seconds each, delivered at one-minute intervals. The ED50 for hydrogen cyanide under similar conditions was attained at 10μg per puff or at concentrations lower than those reported in smoke. Recovery from HCN was more rapid than after cigarette smoke. Smoke from cigarettes with filters containing activated carbon was found to be less ciliatoxic and contained lower levels of HCN and acrolein.