LIVE STREPTOMYCIN-DEPENDENT PASTEURELLA-MULTOCIDA VACCINE FOR PREVENTION OF HEMORRHAGIC SEPTICEMIA

  • 1 January 1978
    • journal article
    • research article
    • Vol. 39  (9) , 1534-1537
Abstract
A type B P. multocida was used for the development of a streptomycin-dependent (StrD) vaccine. P. multocida R-473, a hemorrhagic septicemia strain, was mutagenized with N-methyl-N''-nitro-N-nitrosoguanidine to increase the likelihood of encountering a StrD mutant and was plated on agar containing 400 .mu.g of streptomycin/ml. Replica plating was used to differentiate dependent from resistant colonies. Mice and rabbits were vaccinated with a StrD mutant and 21 days later, were challenge exposed, along with unvaccinated controls, with the wild type R-473. Protection of greater than 4 logs was shown for the vaccinated mice. All vaccinated rabbits were protected and all nonvaccinated controls succumbed to a challenge exposure of 500 or 1000 LD50.

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