Abstract
Tympanocentesis was performed in 50 patients with chronic otitis media; pus was cultured aerobically. Only aerobes were isolated from 21 patients (42%), 3 patients had only anaerobes (Peptococcus sp.), and 25 patients (50%) had aerobes and anaerobes. Only 1 specimen had no growth. There were 68 aerobic isolates. Pseudomonas aeruginosa was recovered in 36 patients (72%); other aerobes commonly recovered included Proteus sp. and Staphylococcus aureus. There were 48 anaerobes isolated. Anaerobic Gram-positive cocci were isolated in 24 instances (21 Peptococcus sp. and 3 Peptostreptococcus sp). Sixteen Bacteroides sp. were recovered, including 7 B. fragilis group and 6 B. melaninogenicus.

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