Structural study of a cell‐wall mannan of Saccharomyces kluyveri IFO 1685 strain

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Abstract
Acetolysis of the cell-wall mannan of Saccharomyces kluyveri under mild conditions, gave fragments with 1–6 mannose residues. The structures of mannopentaose and mannohexaose were determined to be and respectively, by two-dimensional homonuclear Hartmann-Hahn spectroscopy and a sequential NMR assignment method that combines 1H-13C correlated spectroscopy, relayed coherence transfer spectroscopy, 1H-detected heteronuclear multiple-bond connectivity and methylation analysis. The H1 proton chemical shift of a neighboring α-1,2-linked mannose unit of the 3-O-substituted structure was shifted upfield by the addition of a mannose unit to the adjacent 3 O-substituted unit by an α-1,6 linkage. The characteristic H1–H2-correlated cross-peak of the α-1,3-linked mannose unit substituted by a β-1,2 linkage, β1→2Manα1→3, in the mannan of S. kluyveri, as also found by two-dimensional homonuclear Hartmann-Hahn spectroscopy in the mannan of Candida guilliermondii, a pathogenic yeast in man.

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