PURIFICATION OF CANINE PROLACTIN BY PREPARATIVE ISOTACHOPHORESIS
- 31 March 1976
- journal article
- research article
- Published by Bioscientifica in Journal of Endocrinology
- Vol. 69 (1) , 127-132
- https://doi.org/10.1677/joe.0.0690127
Abstract
SUMMARY: Preparative isotachophoresis in polyacrylamide gel using carrier ampholytes as 'spacers' has been used to purify prolactin from canine pituitary extracts. Using Ampholine pH 5–8 as spacers, a prolactin fraction was obtained which was essentially homogeneous as judged by the criteria of disc electrophoresis and isoelectric focusing. Amino acid analysis indicated a close similarity between canine and ovine prolactin. A growth hormone fraction, identified by its electrophoretic mobility, was also obtained although this was heterogeneous in disc electrophoresis at alkaline pH.This publication has 3 references indexed in Scilit:
- Bio-assay of Prolactin. Analysis of the Pigeon Crop-Sac Response to Local Prolactin Injection by an Objective and Quantitative Method1Endocrinology, 1967
- Isoelectric Fractionation, Analysis, and Characterization of Ampholytes in Natural pH Gradients. IV. Further Studies on the Resolving Power in Connection with Separation of Myoglobins.Acta Chemica Scandinavica, 1966
- DISC ELECTROPHORESIS – II METHOD AND APPLICATION TO HUMAN SERUM PROTEINS*Annals of the New York Academy of Sciences, 1964