Composition of Electron Dense Stroma Inclusions in Proplastids of Nicotiana clevelandii×N. glutinosa

Abstract
Proplastids and plastids in meristem cells of the shoot apex from N. clevelandii .times. N. glutinosa contained electron-dense inclusions as shown by transmission electron microscopy. Due to their higher electron-density they can be detected in glutaraldehyde-fixed but unstained material. Application of the method of elementary analysis by X-ray microanalysis (EDAX) showed the presence of both P and Fe in these stroma inclusions. The formation of these inclusions is discussed in relation to plastid and cell development.

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