Photoreactivation of Ultraviolet Irradiation-inactivated RNA from Tobacco Mosaic Virus on White Leaves of a Variegated Mutant of Xanthi Tobacco

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Abstract
It had been observed by one of us (C. A. Knight, unpublished) that green, yellow, and white areas of variegated Xanthi tobacco plants (Nicotiana tabacum L. var. Xanthi nc) yield essentially the same numbers of lesions when inoculated with tobacco mosaic virus (TMV) or its RNA. Since the action spectrum for photoreactivation of viral RNA inactivated by ultraviolet radiation of wave length 254 nm. does not resemble the action spectrum for photosynthesis (Hidalgo-Salvatierra & McLaren, 1969), it seemed possible that photoreactivation (PR) can occur on leaves devoid of chlorophyll. We have tested this hypothesis on white leaves of a variegated mutant of Xanthi tobacco (Burk, Stewart & Dermen, 1964) and have observed a photorecovery of 30 to 35%. Variegated Xanthi plants were grown from seeds kindly provided by Dr H. Dermen. The stems of variegated plants which had been grown to a hieght of 15 to 30 cm. were cut into 6 to 8 cm. pieces and grafted on to normal

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