Prostaglandin Production by Normal and Transformed 3T3 Fibroblasts in Cell Culture

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Abstract
Prostaglandin [PG] E2 and PG F2.alpha. were quantitatively determined in culture media from Balb/c 3T3 [mouse embryo] fibroblasts and 2 virus-transformed derivatives of these cells. Regular and (SV-40)-transformed 3T3 cells produced low and almost identical PG E2 concentrations. The levels were maximal (10 ng/ml) 24 h after planting and decreased during the next 96 h to 2.5 ng/ml. Polyoma-virus-transformed 3T3 cells produced PG E2 continuously for 120 h, giving a final concentration of 270 ng/ml. Fresh medium supplemented with calf serum reinitiated PG production in confluent 3T3 cells. Maximal PG E2 concentrations were obtained between 6 and 18 h after medium change and were proportional to the serum concentration of the medium. Indomethacin (10-6 M) completely inhibited PG E2 production but not PG F2.alpha. production by these cells.