SIMULTANEOUS LOCALIZATION OF 2 DIFFERENT TISSUE ANTIGENS BASED ON THE SILVER INTENSIFIED PAP-DAB AND ON THE TRADITIONAL PAP-DAB METHOD

  • 1 January 1983
    • journal article
    • research article
    • Vol. 31  (4) , 365-369
Abstract
A new immunohistological method was elaborated for the light-microscopic detection of 2 tissue antigens in the same section. One of the antigens was visualized by the Ag-Au intensified peroxidase-antiperoxidase complex (PAP)-diaminobenzidine (DAB) method resulting in a black colored end-product. The other antigen was labeled by the brown reaction-product of the traditional PAP-DAB method. Both reaction-products are stable, electron-dense and they can clearly be distinguished from each other.