The Use of Nucleoside Phosphotransferase and (32P) p-Nitrophenyl Phosphate in the Determination of the 5′-Linked Termini of Ribosomal RNA
Open Access
- 1 July 1969
- journal article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 44 (7) , 978-984
- https://doi.org/10.1104/pp.44.7.978
Abstract
A method for the identification of the 5′-linked termini of ribosomal RNA is described. The method involves the phosphorylation of the nucleosides released from the 5′-linked termini after hydrolysis of the ribonucleic acid chain with alkali. The radioactive 5′-nucleotide derivatives are formed by a nucleoside phosphotransferase mediated phosphoryl transfer from (32P) p-nitrophenyl phosphate to the nucleosides. The sensitivity of the method allows the use of small amounts of ribosomal RNA.Keywords
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