Molecular cloning of a human polypeptide related to yeast sds22, a regulator of protein phosphatase‐1
- 13 November 1995
- journal article
- research article
- Published by Wiley in FEBS Letters
- Vol. 375 (1-2) , 75-78
- https://doi.org/10.1016/0014-5793(95)01180-m
Abstract
Sds22 is a regulatory polypeptide of protein phosphatase-1 that is required for the completion of mitosis in both fission and budding yeast. We report here the cDNA cloning of a human polypeptide that is 46% identical to yeast sds22. The human homolog of sds22 consists of 360 residues, has a calculated molecular mass of 41.6 kDa and shows a tandem array of 11 leucinerich repeat structures of 22 residues. Northern analysis revealed a major transcript of 1.39 kb in all 8 investigated human tissues. sds22 was detected by western analysis in both the cytoplasm and the nucleus of rat liver cells as a polypeptide of 44 kDa.Keywords
This publication has 14 references indexed in Scilit:
- Subunit Structure and Regulation of Protein Phosphatase-1 in Rat Liver NucleiJournal of Biological Chemistry, 1995
- Molecular cloning of cDNA encoding the 110 kDa and 21 kDa regulatory subunits of smooth muscle protein phosphatase 1MFEBS Letters, 1994
- 2.2 Mb of contiguous nucleotide sequence from chromosome III of C. elegansNature, 1994
- Serine/threonine protein phosphatases in the control of cell functionAdvances in Enzyme Regulation, 1994
- Crystal structure of porcine ribonuclease inhibitor, a protein with leucine-rich repeatsNature, 1993
- Protein serine/threonine phosphatases and cell transformationBiochimica et Biophysica Acta (BBA) - Reviews on Cancer, 1993
- Mitotic regulation of protein phosphatases by the fission yeast sds22 proteinCurrent Biology, 1993
- The Structure, Role, and Regulation of Type 1 Protein PhosphatasesCritical Reviews in Biochemistry and Molecular Biology, 1992
- S. pombe gene sds22+ essential for a midmitotic transition encodes a leucine-rich repeat protein that positively modulates protein phosphatase-1Cell, 1991
- An analysis of 5'-noncoding sequences from 699 vertebrate messenger RNAsNucleic Acids Research, 1987