A SIMPLE AND EFFICIENT SQUASH TECHNIQUE FOR SOMATIC CHROMOSOMES OF CRUCIFERS
- 1 June 1973
- journal article
- Published by Canadian Science Publishing in Canadian Journal of Genetics and Cytology
- Vol. 15 (2) , 369-371
- https://doi.org/10.1139/g73-044
Abstract
A simple squash technique has been proposed to study the somatic chromosomes of crucifers. Essentially the technique consists of pretreatment with alpha-bromonaphthelene followed by fixation in methanol, acetic acid (1:1) and Newcomer's fluid, and staining with aceto-orcein. Consistent and excellent preparations have been obtained in rapeseed, cabbage, cauliflower, radish, turnip and Iberis spp.Keywords
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