Method for Determining Oxytocin Concentrations in Unextracted Sera; Characterization in Lactating Cattle
- 1 January 1979
- journal article
- research article
- Published by Frontiers Media SA in Experimental Biology and Medicine
- Vol. 160 (1) , 80-87
- https://doi.org/10.3181/00379727-160-40394
Abstract
A highly sensitive, specific and reproducible double antibody RIA [radioimmunoassay] for measuring oxytocin in unextracted serum of cattle was developed. Serum concentrations of oxytocin were significantly (P < 0.05) elevated from 8.0 .+-. .11 .mu.U[units]/ml to 25 .+-. 7.6 .mu.U/ml (mean .+-. SE) 2 min after commencement of milking. The range of peak oxytocin concentrations seen 2 min after milking in 6 animals was 11.2-65 .mu.U/ml. The T1/2 [half-life] for oxytocin, after milk ejection, was 3.6 min. Simultaneous determinations of oxytocin activity in blood sera and intramammary pressure were monitored in 2 cows before, during and after stimulation of the milk ejection reflex. Ocytocin activity in blood closely paralleled changes in intramammary pressure.This publication has 5 references indexed in Scilit:
- RADIOIMMUNOASSAY OF OXYTOCINJournal of Endocrinology, 1978
- Oxytocin Release During Vaginal Distention in the GoatBiology of Reproduction, 1977
- PLASMA OXYTOCIN AND OXYTOCINASE LEVELS IN 3RD TRIMESTER OF PREGNANCY AND AT LABOR1976
- MILK-EJECTION ACTIVITY (OXYTOCIN) IN THE EXTERNAL JUGULAR VEIN BLOOD OF THE COW, GOAT AND SOW, IN RELATION TO THE STIMULUS OF MILKING OR SUCKLINGJournal of Endocrinology, 1966
- A Sensitivein VitroAssay for Oxytocin1Endocrinology, 1966