MODIFICATION OF VITAMIN-A METABOLISM IN RATS FED A COPPER-DEFICIENT DIET

  • 1 January 1987
    • journal article
    • research article
    • Vol. 57  (3) , 247-252
Abstract
The liver is the main storage site of vitamin A and copper. Inverse relationships between copper and vitamin A liver concentrations have been suggested. We have investigated the consequences of a copper-deficient diet on liver and blood vitamin A storage in Wistar rats. Animals were fed either a copper-deficient diet for 45 days from weaning, or an identical diet containing adequate amounts of copper. Concentrations of vitamin A were determined by isocratic high performance liquid chromatography using UV detection. We have observed in the liver of the rats fed a copper-deficient diet a significantly higher mean level of retinyl esters (148 .+-. 37 .mu.g/g of liver) and retinol (3.3 .+-. 1.4 .mu.g/g of liver) compared to the mean concentration of the retinyl esters (53 .+-. 8.5 .mu.g/g of liver) (p < 0.01) and retinol (1.4 .+-. 0.5 .mu.g/g of liver) (p < 0.01) in controls. Opposite results were observed in the serum of the group fed a copper-deficient diet as these rats had a significantly lower level of retinol (22 .+-. 4 .mu.g/100 ml) compared to the mean concentration in the controls (64 .+-. 20 .mu.g/100 ml) (p < 0.01). These findings suggest that a copper-deficient diet may cause defective transport of vitamin A from liver to blood. This experimental model may be useful to further investigate unsual liver vitamin A and copper concentrations observed in children during various hepatobiliary diseases.

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