SENESCENT ERYTHROCYTES: FACTORS AFFECTING THE AGING OF RED BLOOD CELLS
- 1 January 2002
- journal article
- Published by Taylor & Francis in Immunological Investigations
- Vol. 31 (1) , 41-50
- https://doi.org/10.1081/imm-120003219
Abstract
Human red blood cells (RBC) have a well-defined lifespan of 120 days affected by many cellular parameters. The aim of the present study was to investigate through a functional assay the effect of some factors in the interaction of erythrocytes with monocytes: heat rigidification, equilibration at different pH and desialyzation. We also studied the interaction between stored RBC and peripheral blood monocytes with this functional erythrophagocytosis assay. Blood samples from 30 volunteer donors were investigated. 1) Senescent (Se) and Young (Y) RBC were obtained by differential centrifugation. 2) Erythrocyte suspensions: Aliquots of each sample were subjected to the following treatments: a) Rigidification by heat (RRBC), b) Equilibration at different pH (5.34, 6.30, 7.33, 9.20) and c) Desialyzation with neuraminidase and trypsin. The functional assay was performed incubating monocytes obtained by glass adherence with these suspensions of RBC. Whole blood samples (n=20) were stored during different periods of time (0, 7, 14, 21, 28, 35 and 42 days). The erythrophagocytosis assay was performed during six weeks incubating isologous monocytes with RBC from every unit. Negative and positive controls were performed using non sensitized (NSRBC) and sensitized with IgG anti-RhD (SRBC) red cells. The percentage of active monocytes (AM) obtained were: 1) YRBC: 2.8±0.9 and SeRBC: 17.5±2.1; 2a) RRBC: 3.0±0.9; 2b) 10.9±0.9, 15.5±0.8, 3.1±1.0, 4.0±1.1; 2c) 11.1±1.4 and 3.9±1.0; SRBC 32.1±1.7 and NSRBC: 2.8±1.5. The % of AM with SeRBC was higher (pex vivo model show a significant increase (p<0.001) in the phagocytosis of RBC equilibrated at low pH, desialinized (greater than 80%) with neuraminidase and stored for over 28 days. These factors would be involved in erythrocyte removal via phagocytosis during tissular homeostasis.Keywords
This publication has 16 references indexed in Scilit:
- Sialic Acids in Molecular and Cellular InteractionsPublished by Elsevier ,1997
- Flow cytofluorimetric analysis of young and senescent human erythrocytes probed with lectins. Evidence that sialic acids control their life spanGlycoconjugate Journal, 1995
- Erythrocyte deformability has no influence on the rate of erythrophagocytosis in vitro by autologous human monocytes/macrophagesBritish Journal of Haematology, 1994
- Determinants of red blood cell deformability in relation to cell ageEuropean Journal of Haematology, 1994
- Mechanism of red blood cell aging: Relationship of cell density and cell ageAmerican Journal of Hematology, 1993
- Erythrocyte Aging: Physical and Chemical Membrane ChangesGerontology, 1991
- The Molecular Basis of Erythrocyte ShapeScience, 1986
- Spectrin, human erythrocyte shapes, and mechanochemical propertiesBiophysical Journal, 1986
- The relationship between sialic acid content and peanut agglutinin binding on senescent and enzyme treated human erythrocytesMechanisms of Ageing and Development, 1985
- Senescent cell antigen is immunologically related to band 3.Proceedings of the National Academy of Sciences, 1983