During the period Jan., 1941, through Apr., 1944, 6723 animals were counted as highway victims along 77,000 miles of Nebraska roads. More than 100 spp. were noted and of these amphibia made up 17.4%, reptilia 18.2%, birds 23.5% and mammals 40.5% of the total kill. The amt. of highway travel decreased during this war period, but the 1942 kill increased indicating a general increase in animal numbers. The 3-yr. avg. highway loss was 9.1 animals per 100 miles. 26 spp. made up 88.8% of the total kill. Toads, rabbits, bullsnakes, and pheasants suffered the greatest losses. The greatest number of losses occurred in July, being related to the increased numbers of new animals as well as to the summer increase in highway use. Each of 24 spp. most commonly found on the highways suffered losses in relation to their seasonal activities. Heaviest losses occurred along concrete highways where an animal appeared to have 1/8 the chance of survival that it had crossing other types of road surfaces. Losses were heaviest along roads traversing hardland cultivated soils.