The Effect of Electric Fields on the Dispersion of Oligonucleotides Using a Multi-Point Detection Method in Capillary Gel Electrophoresis

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Abstract
A multi-point detection method is proposed to study the dispersion of oligonucleotides in polyacrylamide gel capillary. In this method a single gel filled capillary is curved into loops, with several detection points aligned with a common detector. In this manner, several electropherograms can be obtained at different migration times during one capillary electrophoresis (CE) run, thus permitting studies of changes in the spatial peak variance σ2 as a function of time. Investigations of polydeoxyadenylic acids in the range of 40–60 bases indicate that the diffusion coefficient of these compounds increases at higher voltages in the polyacrylamide gel-filled capillaries. Using this simple multi-point detection method one can estimate the band broadening originating from sample injection (σ2 inj), which is otherwise difficult to measure.