Binding of phosphorylated and dephosphorylated heavy meromyosin to F‐actin
- 5 January 1987
- journal article
- research article
- Published by Wiley in FEBS Letters
- Vol. 210 (2) , 177-180
- https://doi.org/10.1016/0014-5793(87)81332-7
Abstract
The effect of myosin light chain phosphorylation in skeletal muscle was investigated with respect to the binding affinity of phosphorylated and dephosphorylated heavy meromyosin (HMM) for F-actin in the absence of ATP. For phosphorylated HMM the affinity was 2.5-times weaker in the presence of Ca2+ as in its absence (HMM divalent binding sites saturated only with Mg). For dephosphorylated HMM the reverse was true, the binding being 2.4-times higher in the presence of Ca2+.Keywords
This publication has 27 references indexed in Scilit:
- Urea‐glycerol‐acrylamide gel electrophoresis of acidic low molecular weight muscle proteins: Rapid determination of myosin light chain phosphorylation in myosin, actomyosin and whole muscle samplesElectrophoresis, 1986
- Factors influencing interaction of phosphorylated and dephosphorylated myosin with actinBiochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1985
- Decoration of actin filaments with skeletal muscle heavy meromyosin containing either phosphorylated or dephosphorylated regulatory light chainsBiochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1985
- Phosphorylation kinetics of skeletal muscle myosin and the effect of phosphorylation on actomyosin ATPase activityBiochemistry, 1984
- The regulation of smooth muscle contractile proteinsProgress in Biophysics and Molecular Biology, 1983
- Myosin light chain phosphorylation and phosphorylase a activity in rat extensor digitorum longus muscleBiochemical and Biophysical Research Communications, 1979
- Interaction of myosin subfragments with F-actinBiochemistry, 1978
- Heavy meromyosin binds actin with negative cooperativityBiochemistry, 1978
- Calcium binding to rabbit skeletal myosin under physiological conditionsBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1975
- THE ATTRACTIONS OF PROTEINS FOR SMALL MOLECULES AND IONSAnnals of the New York Academy of Sciences, 1949