HIGH PERFORMANCE LIQUID CHROMATOGRAPHIC DETERMINATION OF ACLACINOMYCIN A AND ITS RELATED COMPOUNDS

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Abstract
A sensitive, simple and selective reverse phase high performance liquid chromatographic method was applied to the determination of aclacinomycin A (ACM) and its metabolites in plasma. The chromatography was performed on a microparticulate phenethyl derivative of silica using a mobile phase of acetonitrile-0.03 M ammonium formate buffer (pH 5.0), (50:50, vol/vol) and monitoring with fluorescence detector (excitation 435 nm-emission 505 nm). High reproducibility and linear calibration curves were obtained from 20-100 ng/ml of ACM and its metabolites in [dog] plasma by use of internal standard methods.