A Highly Sensitive Enzymatic Assay Method for Hypoxanthine Estimation Based on the Use of an Oxygen Electrode
- 1 September 1989
- journal article
- research article
- Published by Taylor & Francis in Analytical Letters
- Vol. 22 (11-12) , 2433-2443
- https://doi.org/10.1080/00032718908052365
Abstract
Using a Clark-type oxygen electrode as sensor, a highly sensitive enzymatic assay method for hypoxanthine was developed, based on xanthine oxidase. The sensitivity of the assay is comparable to that of standard chemiluminescent techniques. The incorporation of sulfite, 0 to 150 mM, allowed hypoxanthine determination over the range of 5 nM to 100 μM. The response time was 4 min or less at 30°C.Keywords
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