Vasoactive intestinal polypeptide modulation of lymphocyte adenylate cyclase.
Open Access
- 1 December 1981
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Immunology
- Vol. 127 (6) , 2551-2554
- https://doi.org/10.4049/jimmunol.127.6.2551
Abstract
Vasoactive intestinal polypeptide (VIP) was found to be a potent stimulator of lymphocyte adenylate cyclase activity. VIP-induced activation of adenylate cyclase was specific for lymphocytes among peripheral blood cells; i.e., VIP did not stimulate the adenylate cyclase activity of neutrophils, monocytes, or platelets. The VIP-induced activation of lymphocyte adenylate cyclase was time, temperature, and concentration dependent. VIP and the GTP analog, Gpp(NH)p, acted synergistically to stimulate lymphocyte adenylate cyclase; stimulation by VIP and PGE1 was additive; and VIP activation was antagonized by somatostatin. VIP-mediated activation of lymphocyte adenylate cyclase was observed in normal human T cells, B cells obtained from a patient with chronic lymphocytic leukemia, and a human T cell culture line. The Raji human B cell culture line did possess adenylate cyclase activity, but this activity was not stimulated by VIP. These results suggest that lymphocytes possess functional receptors for VIP and that this peptide may play a role in modulation of lymphocyte function.This publication has 18 references indexed in Scilit:
- Characterization of a vasoactive intestinal peptide-sensitive adenylate cyclase in rat intestinal epithelial cell membranesBiochimica et Biophysica Acta (BBA) - General Subjects, 1978
- The predominant contribution of platelets to baseline and ascorbate-stimulated increments in cyclic GMP in human mononuclear leukocyte preparationsCellular Immunology, 1978
- Evidence for distinct guanine nucleotide sites in the regulation of the glucagon receptor and of adenylate cyclase activity.Journal of Biological Chemistry, 1977
- Regulation of prostaglandin receptors by prostaglandins and guanine nucleotides in frog erythrocytes.Journal of Biological Chemistry, 1977
- Vasoactive intestinal polypeptide in brain: localization in and release from isolated nerve terminals.Proceedings of the National Academy of Sciences, 1977
- EFFECT OF PHAGOCYTOSIS ON REDUCED SOLUBLE SULFHYDRYL CONTENT OF HUMAN GRANULOCYTES1977
- Intractable watery diarrhea, ganglioneuroblastoma, and vasoactive intestinal peptideThe Journal of Pediatrics, 1976
- Interaction of porcine vasoactive intestinal peptide with dispersed pancreatic acinar cells from the guinea pig. Structural requirements for effects of vasoactive intestinal peptide and secretin on cellular adenosine 3':5'-monophosphate.Journal of Biological Chemistry, 1976
- Regulation of beta-adrenergic receptors by guanyl-5'-yl imidodiphosphate and other purine nucleotides.Journal of Biological Chemistry, 1976
- PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENTJournal of Biological Chemistry, 1951