Kinetic characterization of the acyl-enzyme mechanism for β-lactamase I
- 15 September 1988
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 254 (3) , 923-925
- https://doi.org/10.1042/bj2540923
Abstract
Beta-Lactamase I catalyses the hydrolysis of penicillins by an acyl-enzyme mechanism. A procedure was developed for determining the rate constants for the acylation and deacylation steps for the good substrates benzylpenicillin and phenoxymethylpenicillin; this depends on determining the fraction of enzyme that is present as acyl-enzyme in the steady state.This publication has 15 references indexed in Scilit:
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