Labelling Indices after3H-Thymidine Incorporation during Organ Culture of Duodenal Mucosa in Coeliac Disease
- 1 October 1981
- journal article
- research article
- Published by Taylor & Francis in Scandinavian Journal of Gastroenterology
- Vol. 16 (7) , 921-928
- https://doi.org/10.3109/00365528109181823
Abstract
Incorporation of 3H-thymidine during organ culture of duodenal biopsy specimens from 34 coeliac and 10 non-coeliac patients was studied by autoradiography. High labelling indices were found in flat, coeliac mucosas. Gluten fractions, which provoked histological deterioration during culture, induced labelling of a greater proportion of crypt cells and higher migration rate than parallelly cultured specimens on gluten-free medium. No influences on crypt cell kinetics could be observed after culture with gluten fractions incapable of producing histological damage or with alpha-lactalbumin. In coeliac remission mucosas, labelling indices were at the same level as in non-coeliac biopsies, and no significant effects of gluten were observed. Autoradiography seems to be a fairly sensitive and reliable determinant of gluten toxicity by organ culture in coeliac disease and should supplement the histological appraisal of the biopsies. The increment of labelling indices provoked by gluten exposure seemed not merely to be a consequence of increased desquamation of cells from the biopsy surface but could imply a direct influence of gluten on crypt cell kinetics in coeliac disease.Keywords
This publication has 19 references indexed in Scilit:
- Labelling Indices after3H-Thymidine Incorporation during Organ Culture of Duodenal Mucosa in Coeliac DiseaseScandinavian Journal of Gastroenterology, 1981
- Epidermal DNA synthesis in organ culture expiantsVirchows Archiv B Cell Pathology Including Molecular Pathology, 1979
- Re-evaluation of the techique of organ culture for studying gluten toxicity in coeliac disease.Gut, 1978
- Biosynthesis of brush border glycoproteins by human small intestinal mucosa in organ cultureBiochimica et Biophysica Acta (BBA) - Biomembranes, 1977
- Organ culture of adult guinea-pig intestineCell and tissue research, 1975
- Enzyme changes in human small bowel mucosa during culture in vitroGut, 1974
- An in vitro model of gluten-sensitive enteropathy. Effect of gliadin on intestinal epithelial cells of patients with gluten-sensitive enteropathy in organ culture.Journal of Clinical Investigation, 1974
- Organ culture of adult guinea-pig intestineCell and tissue research, 1974
- The relation between cell division rate and3HTdR incorporation in organ cultureJournal of Cellular Physiology, 1973
- Organ culture of mucosal biopsies of human small intestineJournal of Clinical Investigation, 1969