Batch-To-Batch Variation of Chelex-100 Confounds Metal-Catalysed Oxidation. Leaching of Inhibitory Compounds from A Batch of Chelex-100 and Their Removal by a Pre-Washing Procedure
- 1 January 1995
- journal article
- research article
- Published by Taylor & Francis in Free Radical Research
- Vol. 23 (6) , 533-535
- https://doi.org/10.3109/10715769509065274
Abstract
Removal of adventitious redox-active metals from buffers by treatment with Chelex resin is a widely used procedure in free radical research. Use of a new batch of Chelex-100 resin in our laboratory coincided with a sudden inability to oxidise low-density lipoprotein with copper. We found that copper-mediated oxidation of ascorbate in water treated with the same batch of Chelex was inhibited when compared with untreated water and water treated with a different batch of the resin. Washing the Chelex removed the inhibitory effect suggesting that material was leaching from the resin. The washing procedure for Chelex-100 described is simple and can be scaled up. Oxidation of ascorbate with low concentrations of copper can be used to test the quality of batches of the resin.Keywords
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