Strain Differences in Response of the Mouse Mammary Gland to Hormones In Vitro2
- 1 October 1970
- journal article
- research article
- Published by Oxford University Press (OUP) in JNCI Journal of the National Cancer Institute
- Vol. 45 (4) , 657-675
- https://doi.org/10.1093/jnci/45.4.657
Abstract
Seven strains of mice were examined in regard to the hormonal requirements for the induction of mammary lobuloalveolar differentiation in vitro. The RIII and Is required 5 days of pretreatment in vivo with estradiol and progesterone to show development in vitro; DBA/2, C3H, and BALB/c required 9 days; C57BL and A required 15 days. A greater length of pretreatment was correlated with the absence of the “inherited hormonal influence.” The minimal hormonal requirements in vitro varied with different strains. A concentration of 0.1 μg aldosterone per ml of medium was adequate for all strains except the A, which needed 1.0 μg/ml. Insulin requirements ranged from 0.5–2.5 μg/ml; prolactin and growth hormone requirements, from 0.1–5.0 μg/ml. These in vitro requirements and the “inherited hormonal influence” were not correlated.Keywords
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