Transgenic resistance genes from nepoviruses: Efficacy and other properties

Publisher Website
Google Scholar
Abstract
Evidence of RNA‐RNA recombination or transcapsidation was sought in transgenic tobacco (Nicotiana tabacum L. ‘Xanthi‐nc') containing the 3'‐non coding sequence (1.3 kb) from a birch isolate of cherry leaf roll nepovirus (CLRV). Following mechanical inoculation with a rhubarb isolate of the virus, no evidence of recombination was obtained using polymerase chain reactions whether the transgenic gene produced transcripts in the sense or the antisense orientation. Transgenic tobacco containing the CLRV‐derived sequence (in either orientation) did not lessen the pathological effects of the rhubarb isolate of the virus althdugh there was amelioration of the disease caused by the birch isolate. When expressed as a transgenic gene in tobacco, the capsid coding sequence of arabis mosaic nepovirus (ArMV) lessened infectibility by nematodes carrying that virus. This construct did not influence the pathogenicity of potato virus Yo(PVY0), tobacco rattle tobravirus (PRN), alfalfa mosaic ilarvirus (A1MV), or CLRV, did not alter the levels of their accumulation and did not render their virions more or less prone to recognition by ArMV‐specific antibodies. Uninoculated tobacco expressing the ArMV capsid protein contained ArMV‐like particles which co‐purified with RNA that hybridised with cDNA to tobacco mRNA and to DNA complementary to the capsid coding sequence.

This publication has 53 references indexed in Scilit: