Formation and properties of lactate dehydrogenase inhibitors in NADH.
Open Access
- 1 September 1977
- journal article
- research article
- Published by Oxford University Press (OUP) in Clinical Chemistry
- Vol. 23 (9) , 1576-1580
- https://doi.org/10.1093/clinchem/23.9.1576
Abstract
We describe some characteristics of the mode of formation of inhibitors of lactate dehydrogenase from commercial NADH. Inhibitor formation is time- and concentration-dependent and also varies with the commercial source of NADH. At least two inhibitory components can form in concentrated NADH solutions. One of these can be separated from NADH by chromatography on either diethylaminoethyl-celluose or diethylaminoethyl-Sephadex; the second cannot. The NADH-associated inhibitor appeared to be present in each of the three commercial NADH preparations studied. The 260 nm/340 nm absorbance ratio was of no help in locating this inhibitor during chromatography.This publication has 5 references indexed in Scilit:
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- Detection of inhibitors in reduced nicotinamide adenine dinucleotide by kinetic methods.Clinical Chemistry, 1976
- Purification and analysis of the purity of NADH.Clinical Chemistry, 1976
- Purification and characterization of commercial NADH and accompanying dehydrogenase inhibitorsJournal of Chromatography A, 1976
- CONTROL OF INHIBITORY IMPURITIES IN REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE IN LACTATE DEHYDROGENASE ASSAYS1966