AN IMPROVED METHOD FOR ANALYSIS OF CATECHOLAMINES-GAS-LIQUID CHROMATOGRAPHY (GLC) EQUIPPED WITH ELECTRON-CAPTURE DETECTOR

Open Access
Publisher Website
Google Scholar
Abstract
Analysis of small amounts of catecholamines (CA) in animal tissues by GLC and electron-capture detector (GC-ECD) has been reported. This method allows for adequate separations of a large range of CA and their metabolites and sensitive detection at the picogram level. For the quantitative assay of biogenic CA, the choice of suitable stationary phase, internal standards and acylating agents plays a definite role in the accuracy of the findings. A good separation and sensitive detection of CA and their metabolites was obtained with the use of OV-105 on Chromosorb W as a stationary phase, .alpha.-methyldopamine (.alpha.-MDA) and .alpha.-methylnorepinephrine (.alpha.-MNE) as internal standards for dopamine (DA) and norepinephrine (NE), respectively, and pentafluoropropionic acid anhydride as a fluoroacylating agent. CA [epinephrine, NE and DA] content of rat hypothalamus, adrenal and other tissues is reported.